CauseScience Friday – October 3rd #science #scienceselfie

photo-7

crestwind24 – This morning I am screening through many, many plates of worms. Usually this is very tedious, but today it is very exciting because I am looking for worms that have had their genomes edited using the CRISPR/Cas9 system!!! The CRISPR system allows scientists to make specific genetic changes in the actual genes of bacteria, cells, and animals. In the case of C. elegans, the CRISPR system allows scientists to target genes within the genome to mutate them, change them, delete them, or add things to them. It is extremely powerful, and hopefully I will find some worms that have the genetic edit that I designed!! Cross your fingers for me!

2014-10-03 11.18.26

psgurel– Speaking of tedious, I am in the final steps of expressing and purifying protein.  Purified protein is a requirement for a variety of biochemical experiments (like the kinetic assay or analytical ultracentrifugation I mentioned earlier). This whole process takes 5 days for the protein I’m expressing, where I’ve had to: grow and express my protein in E. Coli (E. Coli is typically used for protein expression because they grow very fast and yield a lot of protein), lyse the bacterial cells, harvest the protein through various methods of chromatography, concentrate my protein, then store it appropriately.  Here I am posing with the FPLC, in the final step of purifying my protein.  Looks like I got a good yield, yippee!

Check out our past CauseScience Friday posts! 

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s